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KMID : 0352519820190030617
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Korea Univercity Medical Journal
1982 Volume.19 No. 3 p.617 ~ p.623
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Electron-Microscopic Studies on the Effect of Praziquantel to Paragonimus westermani
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曲華星/Chiu, Hwa-Shing
金洙鎭/林漢鍾/Kim, Soo-Jin/Rim, Han-Jong
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Abstract
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The present study was undertaken to observe the morphological changes of Paragonimus westermani which was obtained from experimentally infected dogs after treatment with praziquantel (Biltricide) a newly developed anthelmintic agent. For this study 6 dogs were artificially infected with metacercariae isolated from crayfish, Cambaroides similis. After 3 months of infection, each two dogs were given praziquantel 3 x 25 mg/kg x 3 days and 3 x 50 mg/kgx 3 days respectively. After each dog was autopsied at 24 hours and on 7 days after the last medication, the lesions by the Paragonimus worms were observed in the lungs of the dogs. With the collected worms from the worm-cysts, the fine structures were studied by means of scanning and transmission electron microscope (TEM). The findings of the observation were compared with those of untreated worms obtained from two control dogs.
The results are as follows:
1. With the above two doses, no living worm was found in the worm-cysts in the lungs of treated dogs. The most of worm-cysts were filled with the necrotic masses with many eggs of P. westermani. However partly degenerated worms could be collected from a dog autopsied at 24 hours after the last day of medication (praziquantel 3 x 25 mg/kgx 3 days).
2. By scanning electron microscopic (SEM) observation, the tegument of the anterior part of the worm in which many spines were distributed had nearly its normal shape, but that of the posterior part from the ventral sucker in which little spines were distributed had exfoliated completely from the worm tissues.
3. It was also observed by SEM that the bleb like structures were formed at the papillae near two suckers and the host cells of the defense system attached to the tegumental
vacuoles caused by bursting of the blebs.
4. Even in the spiny tegumental surface of the worm it appeared as almost normal in shape by SEM, and the bubble-like vacuoles and vesicles in the tegumental syncytium were observed by TEM along the basement membrane being detached from the circular muscles of the worm.
5. It was also observed that many host cells (granulocytes) invaded to the exposed muscular layer of the worm after exfoliation of the tegument and the interior of the parasites was destroyed by the host cells.
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